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Richard Shim
Mr. Lakey
11/09/12
Catalase-H2O2 AP Lab

1)      Time Interval (seconds)
| 0 to 10| 10 - 30| 30 - 60| 60 - one hundred twenty| 120 - 180| 180 - 360|
Rate (µmoles/sec)| 9.45| 12.6| 1.05| 1.575| 4.725| .175|

2) The come out of H2O2 consumption is highest between 10 and 30 seconds interval, because not much of the H2O2 have save to be spoild. There are a lot of H2O2 left, and so making it easier for the enzymes to bump into H2O2 and react with them. However, the reason why the rate is not the highest in 0 to 10 seconds interval is because enzymes look time to break down the molecules.

3) The rate of H2O2 consumption is low between 180 and 360 seconds interval. The rate is the slowest because the enzymes have already broken down most of the H2O2 molecules. This makes harder for the enzymes to break down the rest, because on that point are barely any left.

4) Sulfuric acid was added at specific times to change the pH level of the enzyme. This causes the enzyme to be denatured and stop chemical chemical reactions to further occur. The change of pH disrupts the fragile hydrogen bond in the secondary twist of the enzyme, also resulting the change in the tertiary structure of the enzyme.



When the enzyme is deformed, it does not match up with the molecules anymore; as a result, the reaction stops.

5) The catalse was kept in an ice-water bath, in raise to prevent the enzymes to decompose quickly. Under a colder temperature, the decomposition rate decreases substantially, which allows the enzymes to be uphold longer. However, when the temperature becomes too cold, the enzymes will get denatured, thus stopping the gas pedal reaction with H2O2.

6) First, put the catalase and hydrogen peroxide in the beaker, meet amount as the initial experiment. Then, in order to modify the pH level, instead of applying sulfuric acid, any other materials bay window go in. Everything but the pH level will pillow the same. Any pH level from gastric acid to lye bath be applied...If you want to get a full essay, order it on our website: Orderessay



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